Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.

@article{Yang2002ShortRD,
  title={Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells.},
  author={Dun Yang and Frank Buchholz and Zhongdong Huang and Andrei Goga and C Chen and Frances M. Brodsky and J. Michael Bishop},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 15},
  pages={9942-7}
}
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing capacities, RNA interference with synthetic siRNA is inefficient and cost intensive, especially for functional genomic studies. Here we report the use of Escherichia coli RNase III to cleave double-stranded RNA (dsRNA) into endoribonuclease-prepared siRNA (esiRNA) that can target multiple sites within an… CONTINUE READING
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