Short Interfering RNA Strand Selection Is Independent of dsRNA Processing Polarity during RNAi in Drosophila

@article{Preall2006ShortIR,
  title={Short Interfering RNA Strand Selection Is Independent of dsRNA Processing Polarity during RNAi in Drosophila},
  author={Jonathan B. Preall and Zhengying He and Jeffrey M. Gorra and Erik J Sontheimer},
  journal={Current Biology},
  year={2006},
  volume={16},
  pages={530-535}
}
Short interfering RNAs (siRNAs) guide mRNA cleavage during RNA interference (RNAi). Only one siRNA strand assembles into the RNA-induced silencing complex (RISC), with preference given to the strand whose 5' terminus has lower base-pairing stability. In Drosophila, Dcr-2/R2D2 processes siRNAs from longer double-stranded RNAs (dsRNAs) and also nucleates RISC assembly, suggesting that nascent siRNAs could remain bound to Dcr-2/R2D2. In vitro, Dcr-2/R2D2 senses base-pairing asymmetry of synthetic… CONTINUE READING

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