Shigella dysenteriae 1 cytotoxin: periplasmic protein releasable by polymyxin B and osmotic shock.

@article{DonohueRolfe1983ShigellaD1,
  title={Shigella dysenteriae 1 cytotoxin: periplasmic protein releasable by polymyxin B and osmotic shock.},
  author={Arthur Donohue-Rolfe and G T Keusch},
  journal={Infection and immunity},
  year={1983},
  volume={39 1},
  pages={270-4}
}
Treatment of Shigella dysenteriae 1 either with the antibiotic polymyxin B or by osmotic shock resulted in the release of 80 to 90% of the cytotoxin activity of the organism. Under the conditions employed, the release of toxin activity was accompanied by the appearance of a periplasmic enzyme, 5'-nucleotidase. There was no significant release of cytoplasmic… CONTINUE READING