Shedding New Light on Lipid Biology with CARS Microscopy

Abstract

Despite the ubiquitous roles of lipids in biology, the detection of lipids has relied on invasive techniques, population measurements, or non-specific labeling. Such difficulties can be circumvented by a label-free imaging technique known as coherent anti-Stokes Raman (CARS) microscopy which is capable of chemically selective, highly sensitive, and high-speed imaging of lipid-rich structures with submicron 3-D spatial resolution. We review the broad applications of CARS microscopy to studies of lipid biology in cell cultures, tissue biopsies, and model organisms. Recent technical advances, limitations of the technique, and perspectives are discussed. Corresponding author: Ji-Xin Cheng, jcheng@purdue.edu by gest, on O cber 0, 2017 w w w .j.org D ow nladed fom

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Cite this paper

@inproceedings{Le2010SheddingNL, title={Shedding New Light on Lipid Biology with CARS Microscopy}, author={Thuc T. Le and Shuhua Yue and Ji-Xin Cheng}, year={2010} }