Shear-induced self-assembly of native silk proteins into fibrils studied by atomic force microscopy.

@article{Greving2012ShearinducedSO,
  title={Shear-induced self-assembly of native silk proteins into fibrils studied by atomic force microscopy.},
  author={Imke Greving and Minzhen Cai and Fritz Vollrath and Hannes Christian Schniepp},
  journal={Biomacromolecules},
  year={2012},
  volume={13 3},
  pages={
          676-82
        }
}
Noncontact mode atomic force microscopy was used to investigate native silk proteins prepared in different ways. Low protein concentrations revealed that single protein molecules exhibit a simple, round shape with apparent diameters of 20-25 nm. Shearing the native protein solutions after extraction from the gland and prior to drying led to a beads-on-a-string assembly at the nanometer scale. Protein concentration had a significant effect on the morphology of the protein assemblies. At higher… CONTINUE READING
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