There are disputed claims about the differences of the size of the human corpus callosum in men and women and the relationship of any such differences to gender differences in human behaviour and cognition. There is scientific dispute not only about the implications of anatomical difference, but whether such a difference actually exists. The corpus callosum is the largest commissure of the brain. It is seen as a thick, curved white band on medial surface of sagittaly bisected brain. The Corpus callosum is 10 cm long, and has 4 parts i.e. rostrum, genu, trunk and splenium. The present study was carried out on 120 individuals (78 males & 42 females) between the age group of 1-85 years of age who visited the OPD of Department of Radio-diagnosis, Sardar Vallabh Bhai Patel Hospital and NMC Sky Imaging Centre, LLRM Medical College, Meerut. MRI scans were studied for splenial thickness of corpus callosum in mid-sagittal plane & comparison was done in males & females by using two way ANOVA procedure. No significant sexual dimorphism in splenial thickness was found in the present study.