Sexing of porcine embryo by in situ hybridization using chromosome Y- and 1-specific DNA probes.

@article{Kawarasaki2000SexingOP,
  title={Sexing of porcine embryo by in situ hybridization using chromosome Y- and 1-specific DNA probes.},
  author={Tatsuo Kawarasaki and Kazuya Matsumoto and Mikio Chikyu and Yasuharu Itagaki and Atsushi Horiuchi},
  journal={Theriogenology},
  year={2000},
  volume={53 7},
  pages={1501-9}
}
This study was carried out to determine if a rapid, simultaneous detection system using chromosome Y- and 1-bearing boar spermatozoa was applicable for sexing embryos. Porcine embryos were recovered from gilts and sows 4 to 6 d after mating, and whole embryos or biopsy cells were mounted on a glass slide with a small amount of fixative (methanol: acetic acid: distilled water = 9:1:4). The samples were then stained by means of a fluorescence in situ hybridization (FISH) procedure developed… CONTINUE READING
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