Sexing of murine and bovine embryos by developmental arrest induced by high-titer H-Y antisera.

@article{Ramalho2004SexingOM,
  title={Sexing of murine and bovine embryos by developmental arrest induced by high-titer H-Y antisera.},
  author={Margarita Ramalho and Joaquim Mansano Garcia and C{\'e}sar Roberto Esper and R Vantini and Beatriz da Costa Aguiar Alves and I L Almeida Junior and V F M Hossepian de Lima and Carlos Alberto Moreira-Filho},
  journal={Theriogenology},
  year={2004},
  volume={62 9},
  pages={1569-76}
}
Murine and bovine embryos at the late morula stage were cultured in medium containing high-titer rat H-Y antisera. After 12h of incubation, embryos blocked at the late morulae stage were classified as males and those at the blastocyst stage were classified as females. Sexing of murine embryos by PCR and cytogenetics revealed that 83% of the embryos classified as males and 82% of those classified as females had their sex correctly predicted (P < 0.05). Bovine embryos were transferred to… CONTINUE READING

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