Sexing of mouse preimplantation embryos by detection of Y chromosome-specific sequences using polymerase chain reaction.

@article{Kunieda1992SexingOM,
  title={Sexing of mouse preimplantation embryos by detection of Y chromosome-specific sequences using polymerase chain reaction.},
  author={Tetsuo Kunieda and M Xian and Etsuko Kobayashi and Tomonori Imamichi and Kazuo Moriwaki and Yutaka Toyoda},
  journal={Biology of reproduction},
  year={1992},
  volume={46 4},
  pages={
          692-7
        }
}
Detection of genes known to be present on the mammalian Y chromosome was adapted for sexing mouse early embryos using the polymerase chain reaction (PCR) method. Sry and Zfy genes located in the sex-determining region of the Y chromosome were chosen for Y-specific target sequences, and DXNds3 sequence on the X chromosome was chosen for control. The two-step PCR method using two pairs of primers for each of the target sequences was employed for detecting the sequences. When DNAs of male and… CONTINUE READING

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