Sexing Goat Embryos by PCR Amplification of X- and Y- chromosome Specific Sequence of the Amelogenin Gene*


The objective of this study was to develop a simplified, efficient, and accurate protocol for sexing goat embryos. Based on the amelogenin gene located on the conservation region of Xand Ychromosomes, a pair of primers was utilized and the system of PCR was established to amplify a 262 bp fragment from the Xchromosome in female goats, and a 262 bp fragment… (More)


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