Sex-specific transcriptional regulation of the C. elegans sex-determining gene her-1

  title={Sex-specific transcriptional regulation of the C. elegans sex-determining gene her-1
  author={Carol Trent and Beverly A. Purnell and Sheri Gavinski and J M Hageman and Caroline Chamblin and William Barry Wood},
  journal={Mechanisms of Development},
Evidence for multiple promoter elements orchestrating male-specific regulation of the her-1 gene in Caenorhabditis elegans.
Results suggest that normal repression of transcription from P1 in XX animals may involve cooperative interaction with sequences in the P2 region, and potential cis-regulatory sites required for the putative titration of sdc gene products by sequences in arrays are defined.
Sequenced alleles of the Caenorhabditis elegans sex-determining gene her-1 include a novel class of conditional promoter mutations.
Of the 32 extant mutant alleles, 30 cause partial or complete loss of masculinizing function (lf), while 2 are gain-of-function (gf) alleles resulting in abnormal masculinization of XX animals, and six are in the promoter region, including the two gf mutations.
xol-1, the master sex-switch gene in C. elegans, is a transcriptional target of the terminal sex-determining factor TRA-1
A consensus TRA-1 binding site in the regulatory region of xol-1, the master switch gene controlling sex determination and dosage compensation is identified, implying the existence of a regulatory feedback loop within the C. elegans sex-determination and dosage-compensation cascade that ensures the accurate dose of X-linked genes in cells destined to adopt hermaphrodite fate.
A novel regulatory mutation in the C. elegans sex determination gene tra-2 defines a candidate ligand/receptor interaction site.
P phenotypic characterisation of sexually transformed XO tra-2(eg) hermaphrodites reveals that their fertility is strongly affected by dosage compensation mutations, suggesting that dosage compensation plays a role in normal gametogenesis.
Function of the her-1 gene is required for maintenance of male differentiation in adult tissues of C. elegans.
It is shown that when XO males reared at a permissive temperature are shifted as adults to a nonpermissive temperature, they initiate vitellogenin synthesis in the intestine and oocyte production in the germline, concluding that sexual differentiation of the intestinal and germline is plastic.
Germ-line regulation of the Caenorhabditis elegans sex-determining gene tra-2.
The Caenorhabditis elegans sex-determining gene tra-2 promotes female development of the XX hermaphrodite soma and germ line. We previously showed that a 4.7-kb tra-2 mRNA, which encodes the membrane
Evidence from mosaic analysis of the masculinizing gene her–1for cell interactions inC. eleganssex determination
The results suggest that her-l is expressed in many, possibly all, cells and that its gene product can function non-autonomously through cell interactions to determine male sexual development.
Somatic sex determination.
  • D. Zarkower
  • Biology
    WormBook : the online review of C. elegans biology
  • 2006
C. elegans occurs in two natural sexes, the XX hermaphrodite and the XO male, which differ extensively in anatomy, physiology, and behavior. All somatic differences between the sexes result from the
Recruitment of C. elegans dosage compensation proteins for gene-specific versus chromosome-wide repression
Dpy-21 mutations, shown here to be null, cause elevated X-linked gene expression in XX animals, but unlike mutations in other dosage compensation genes, they do not cause extensive XX-specific lethality or disrupt the stability or targeting of the dosage compensation complex to X.


Molecular cloning and transcript analysis of fem-3, a sex-determination gene in Caenorhabditis elegans.
The fem-3 gene is required for specification of the male fate in the nematode Caenorhabditis elegans: XO males need fem-3 for male differentiation in both soma and germ line; XX hermaphrodites need
The Caenorhabditis elegans gene sdc-2 controls sex determination and dosage compensation in XX animals.
A model in which sdc-2 is involved in the coordinate control of both sex determination and dosage compensation in XX animals and acts in the regulatory hierarchy at a step prior to the divergence of the two pathways is proposed.
The role of sdc-1 in the sex determination and dosage compensation decisions in Caenorhabditis elegans.
Analysis of 14 new sdc-1 alleles suggests that the phenotypes resulting from the lack of sDC-1 function are an incompletely penetrant sexual transformation of XX animals toward the male fate, and increased levels of X-linked gene transcripts in XX animals, correlated with XX-specific morphological defects but not significant XX- specific lethality.
More sex-determination mutants of Caenorhabditis elegans.
The epistatic relationships between tra and her genes are used to deduce a model for the action of these genes in controlling sex determination, and the her-2 mutation may result in constitutive expression of tra-1.
Sex determination in the nematode C. elegans: analysis of tra-3 suppressors and characterization of fem genes.
All three fem genes are essential for male development and appear to have a dual role in promoting spermatogenesis and repressing tra-1 activity; the maternal contribution of fem gene products may be inactivated in XX animals by a posttranscriptional mechanism.
A novel dominant transformer allele of the sex-determining gene her-1 of Caenorhabditis elegans.
The existence of dominant and recessive alleles that cause opposite phenotypic transformations demonstrates that the her-1 gene acts to control sexual identity in C. elegans.