Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling

@article{Anslinger2005SexspecificFL,
  title={Sex-specific fluorescent labelling of cells for laser microdissection and DNA profiling},
  author={Katja D Anslinger and Birgit Bayer and B. Mack and Wolfgang Eisenmenger},
  journal={International Journal of Legal Medicine},
  year={2005},
  volume={121},
  pages={54-56}
}
Sex-specific isolation of cells from mixtures would greatly facilitate forensic casework. Thus, male and female cell mixtures were marked with a fluorescent X/Y-probe CEP X SpectrumOrange/Y SpectrumGreen DNA probe kit for fluorescence in situ hybridization, and single cells were isolated via laser microdissection (LMD). DNA profiling of LMD isolated, hybridized cells showed usable short tandem repeat profiles for at least 20 cells, which are comparable with results from other studies. To… CONTINUE READING

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