Sex-sorting sperm using flow cytometry/cell sorting.


The sex of mammalian offspring can be predetermined by flow sorting relatively pure living populations of X- and Y-chromosome-bearing sperm. This method is based on precise staining of the DNA of sperm with the nucleic acid-specific fluorophore, Hoechst 33342, to differentiate between the subpopulations of X- and Y-sperm. The fluorescently stained sperm are… (More)


Cite this paper

@article{Garner2013SexsortingSU, title={Sex-sorting sperm using flow cytometry/cell sorting.}, author={Duane L. Garner and Katherine M. Evans and George E. Seidel}, journal={Methods in molecular biology}, year={2013}, volume={927}, pages={279-95} }