Sex ratio of early embryos fertilized in vitro with spermatozoa separated by percoll.

@article{Iwasaki1988SexRO,
  title={Sex ratio of early embryos fertilized in vitro with spermatozoa separated by percoll.},
  author={Shintaro Iwasaki and Yasuo Shioya and Hiroshi Masuda and A L Hanada and Tomomi Nakahara},
  journal={Theriogenology},
  year={1988},
  volume={30 6},
  pages={1191-8}
}
Early bovine embryos were obtained by in vitro fertilization and sexing carried out by chromosome analysis. Separation of bovine X- and Y-bearing spermatozoa was performed using Percoll density gradient centrifugation and the enrichment of X-sperm proportion was investigated. Through treatment with vinblastin sulfate and podophyllotoxin, 880 (48.6%) of 1812 embryos at two- to seven-cell stages at 48 to 53 h after sperm-egg incubation produced metaphase spreads, and 399 (45.3%) of these were… CONTINUE READING