Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency.

@article{Johnson1999SexPH,
  title={Sex preselection: high-speed flow cytometric sorting of X and Y sperm for maximum efficiency.},
  author={Lawrence A. Johnson and G. Rickey Welch},
  journal={Theriogenology},
  year={1999},
  volume={52 8},
  pages={1323-41}
}
Sex preselection that is based on flow-cytometric measurement of sperm DNA content to enable sorting of X- from Y-chromosome-bearing sperm has proven reproducible at various locations and with many species at greater than 90% purity. Offspring of the predetermined sex in both domestic animals and human beings have been born using this technology since its introduction in 1989. The method involves treating sperm with the fluorescent dye, Hoechst 33342, which binds to the DNA and then sorting… CONTINUE READING