Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Leporinus elongatus

@article{Nakayama1994SexCP,
  title={Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Leporinus elongatus},
  author={Ichiro Nakayama and Fausto Foresti and Rita Tewari and Manfred Schartl and Daniel Chourrout},
  journal={Chromosoma},
  year={1994},
  volume={103},
  pages={31-39}
}
In order to study the divergence of teleost sex chromosomes, subtractive cloning was carried out between genomic DNA of males and females of the rainbow trout (XX/XY) and of Leporinus elongatus (ZW/ZZ). Inserts cloned in a plasmid vector were individually tested on Southern blots of DNA of males and females for sex specificity. No sex-specific insert was obtained from trout, but two out of ten inserts cloned from L. elongatus showed sex-specific patterns in this species: one corresponds to a… CONTINUE READING

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