Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis

  title={Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis},
  author={Brenda Ariza{\'i} Alvarez-Sandoval and Linda R. Manzanilla and Rafael Montiel},
  booktitle={PloS one},
Sex identification in ancient human remains is a common problem especially if the skeletons are sub-adult, incomplete or damaged. In this paper we propose a new method to identify sex, based on real-time PCR amplification of small fragments (61 and 64 bp) of the third exon within the amelogenin gene covering a 3-bp deletion on the AMELX-allele, followed by a High Resolution Melting analysis (HRM). HRM is based on the melting curves of amplified fragments. The amelogenin gene is located on both… CONTINUE READING
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