Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis

@inproceedings{AlvarezSandoval2014SexDI,
  title={Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis},
  author={Brenda Ariza{\'i} Alvarez-Sandoval and Linda R. Manzanilla and Rafael Montiel},
  booktitle={PloS one},
  year={2014}
}
Sex identification in ancient human remains is a common problem especially if the skeletons are sub-adult, incomplete or damaged. In this paper we propose a new method to identify sex, based on real-time PCR amplification of small fragments (61 and 64 bp) of the third exon within the amelogenin gene covering a 3-bp deletion on the AMELX-allele, followed by a High Resolution Melting analysis (HRM). HRM is based on the melting curves of amplified fragments. The amelogenin gene is located on both… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 7 times over the past 90 days. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.
5 Citations
103 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 103 references

Proyecto ‘‘Teotihuacan: elite y gobierno’’, 1997–2005

  • LR Manzanilla
  • Manzanilla LR, editor. Estudios arqueométricos…
  • 2014
1 Excerpt

Accurate sex identification of ancient human remains using DNA shotgun sequencing

  • P Skoglund, J Storå, A Götherström, M Jakobsson
  • J Archaeol Sci
  • 2013
1 Excerpt

Similar Papers

Loading similar papers…