Set-up of large laboratory-scale chromatographic separations of poly(ethylene glycol) derivatives of the growth hormone-releasing factor 1-29 analogue.

@article{Piquet2002SetupOL,
  title={Set-up of large laboratory-scale chromatographic separations of poly(ethylene glycol) derivatives of the growth hormone-releasing factor 1-29 analogue.},
  author={Gilles Piquet and Michele Gatti and Luca Barbero and Silvio Traversa and Photomultipliers M. Caccia and Pierandrea Esposito},
  journal={Journal of chromatography. A},
  year={2002},
  volume={944 1-2},
  pages={141-8}
}
In this paper we report the scale-up of the purification of poly(ethylene glycol) (PEG) derivatives of the growth hormone-releasing factor 1-29, from laboratory scale (100 mg of bulk starting material) to larger scale (3 g of bulk), through the use of a cation-exchange TSK-SP-5PW chromatographic column. A one-step purification process capable of purifying large amounts of mono-PEGylated GRF species from the crude reaction mixture was developed. A simple, straightforward stepwise gradient… CONTINUE READING