Serum trypsin, alpha-amylase and lipase during bombesin stimulation in normal subjects and patients with pancreatic insufficiency.

@article{Hafkenscheid1984SerumTA,
  title={Serum trypsin, alpha-amylase and lipase during bombesin stimulation in normal subjects and patients with pancreatic insufficiency.},
  author={J. Hafkenscheid and M. Hessels and J. Jansen and C. Lamers},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1984},
  volume={136 2-3},
  pages={
          235-40
        }
}
The effect of infusion of bombesin (60 pmol/kg 20 min) on pancreatic enzymes in serum was studied in 13 normal subjects and 12 patients with pancreatic insufficiency. In normal subjects administration of bombesin induced large increases in serum trypsin (p less than 0.01), while serum total alpha-amylase and pancreatic alpha-amylase did not change and serum lipase showed only a modest rise (0.01 less than p less than 0.05). Patients with pancreatic insufficiency had significantly lower serum… Expand
Effect of secretin upon the levels of pancreatic enzymes in blood serum.
TLDR
Using the peak activities of the enzymes, only trypsin distinguishes healthy control subjects from mild to moderate chronic pancreatitis, and is proposed to put into clinical practice this serum provocative test to evaluate the functional damage of an established Chronic pancreatitis. Expand
Raised serum concentrations of pancreatic enzymes in smokers.
TLDR
The results of Dubick et al' and Balldin et aP indicate that in normal non-smokers there is no enzyme response after secretin for immunoreactive cationic trypsinogen, P-isoamylase, and/or immunore active elastase-2 in contrast with the smoking group. Expand
Klinische Relevanz der immunchemischen Bestimmung der Pankreas-Lipase bei chronischer Pankreatitis
TLDR
The organ specificity of a recently introduced immunochemical method of determining pancreatic lipase is guaranteed and specificity is limited, since an abnormal serum lipase increase after stimulation is found in 20% of controls. Expand
Effect of Bombesin and Cholecystokinin on Plasma Immunoreactive Trypsin in Humans
TLDR
It is concluded that the stimulatory effect of bombesin on plasma immunoreactive trypsin is not mediated by CCK. Expand
Role of intraduodenal proteases in plasma cholecystokinin and pancreaticobiliary responses to protein and amino acids.
TLDR
This study shows that appropriate digestion of protein is required to stimulate plasma cholecystokinin release, gallbladder emptying, and pancreatic enzyme secretion in humans. Expand
Evaluation of Tests of Exocrine and Endocrine Pancreatic in Function in Older Patients with Cystic Fibrosis
TLDR
Serum levels of trypsin, pancreatic isoamylase and lipase, and the urinary excretion of PABA showed significant negative correlations with fecal fat excretion, and these tests were the most sensitive tests for severe exocrine pancreatic insufficiency. Expand
Improvement of fecal fat excretion after addition of omeprazole to pancrease in cystic fibrosis is related to residual exocrine function of the pancreas
TLDR
The addition of omeprazole to pancrease is most successful in CF patients with residual pancreatic function, determined by urinary PABA excretion or incremental PP. Expand
Biochemical tests in the diagnosis of chronic pancreatitis and in the evaluation of pancreatic insufficiency.
TLDR
Biochemical tests to detect pancreatic insufficiency and to identify pancreatic disease as the cause of steatorrhoea and the role of various biochemical procedures to evaluate the efficacy of pancreatic enzyme replacement therapy are described. Expand
The Assessment of Gastrointestinal Function
TLDR
This chapter focuses on the assessment of gastrointestinal function, and the main anatomical regions of the stomach, the cardia, fundus, body, antrum, and pylorus have different physiological functions. Expand
The endocrine secretion of mammalian digestive enzymes by exocrine glands.
TLDR
A variety of evidence suggests that the endocrine secretion of digestive enzymes is a normal occurrence that can be of substantial magnitude in healthy individuals, is responsive to various physiological stimuli, and is distinct from exocrine secretion. Expand
...
1
2
...

References

SHOWING 1-10 OF 13 REFERENCES
Changes in serum total and pancreatic amylase after administration of secretin and cholecystokinin-pancreozymin in patients with early and advanced chronic pancreatitis, and in normal subjects
TLDR
In both disease groups, the measurement of serum pancreatic amylase was at least as accurate as duodenal intubation in detecting pancreatic dysfunction, and in the group with early disease there appeared to be a clear advantage for the evocative serum test. Expand
External pancreatic secretion after bombesin infusion in man.
TLDR
In man, bombesin exerts an effect on pancreatic secretion that mimics the effect of CCK-PZ, thus confirming the results obtained in the experimental animal. Expand
Plasma trypsin in chronic pancreatitis and pancreatic adenocarcinoma.
TLDR
In contrast to exocrine deficient chronic pancreatitis, other malabsorptive conditions associated with steatorrhoea (active coeliac disease and acute tropical sprue) demonstrated mean fasting trypsin concentrations similar to controls. Expand
DIAGNOSTIC IMPORTANCE OF CHANGES IN CIRCULATING CONCENTRATIONS OF IMMUNOREACTIVE TRYPSIN
A specific and sensitive radioimmunoassay (R.I.A.) has been developed which makes possible the determination of serum or plasma trypsin concentrations despite the presence of trypsin inhibitors,Expand
Differential serum amylase determination by use of an inhibitor, and design of a routine procedure.
TLDR
A new method for measuring pancreatic and salivary-type amylases in serum that requires no electrophoresis or chromatography is described, and the increase in serum amylase activity in pancreatitis was shown to be attributable to the pancreatic component. Expand
Origin of circulating serum immunoreactive trypsin in man
TLDR
It is concluded that circulating serum trypsin is derived, at least in part, from intestinal reabsorption. Expand
Basal and stimulated serum immunoreactive trypsin in normal subjects.
TLDR
No correlation was found between age and serum IRT levels, nor were any statistical differrences observed in IRT concentration between the two sexes, and only rapid i.v. injection of secretin was able to induce a significant rise of IRT over basal levels in healthy subjects. Expand
Bombesin: action on gut hormones and calcium in man.
TLDR
Synthetic bombesin was infused at low doses in six male volunteers to investigate its effect in man, and concentrations achieved were low enough to indicate a possible physiological role for the endogenous peptide. Expand
In vitro action of bombesin and bombesin-like peptides on amylase secretion, calcium efflux, and adenylate cyclase activity in the rat pancreas: a comparison with other secretagogues.
TLDR
Bombesin, bombesin-NP, and litorin did not activate adenylate cyclase in a purified pancreatic plasma membrane preparation, whereas caerulein and secretin increased this activity 20 and 16-times, respectively... Expand
A new and rapid method for the clinical determination of alpha-amylase activities in human serum and urine. Optimal conditions.
TLDR
A new type of α-amylase starch substrate rendered insoluble by cross-linking and coloured by a dye marker, was shown to be hydrolyzed by α-Amylase from Hyland standard serum, Versatol-E and Enzatrol, and it was possible, with considerable accuracy, to determine α- amylase over more than a thousandfold range of enzyme concentration. Expand
...
1
2
...