Serum-starved apoptotic fibroblasts reduce blastocyst production but enable development to term after SCNT in cattle.

@article{Miranda2009SerumstarvedAF,
  title={Serum-starved apoptotic fibroblasts reduce blastocyst production but enable development to term after SCNT in cattle.},
  author={Moys{\'e}s dos Santos Miranda and Fabiana Fernandes Bressan and Karina Gottardello Zecchin and Anibal E Vercesi and L{\'i}gia Garcia Mesquita and Giovana Krempel Fonseca Merighe and William Allan King and Ot{\'a}vio Mitio Ohashi and Jos{\'e} Rodrigo Valim Pimentel and Felipe Perecin and Fl{\'a}vio Vieira Meirelles},
  journal={Cloning and stem cells},
  year={2009},
  volume={11 4},
  pages={565-73}
}
Cell cycle synchronization by serum starvation (SS) induces apoptosis in somatic cells. This side effect of SS is hypothesized to negatively affect the outcome of somatic cell nuclear transfer (SCNT). We determined whether apoptotic fibroblasts affect SCNT yields. Serum-starved, adult, bovine fibroblasts were stained with annexin V-FITC/propidium iodide to allow apoptosis detection by flow cytometry. Positive and negative cells sorted by fluorescence activated cell sorting (FACS) and an… CONTINUE READING