Serum-induced basophil CD63 expression by means of a tricolour flow cytometric method for the in vitro diagnosis of chronic urticaria.

@article{Frezzolini2006SeruminducedBC,
  title={Serum-induced basophil CD63 expression by means of a tricolour flow cytometric method for the in vitro diagnosis of chronic urticaria.},
  author={Alessandra Frezzolini and Alessia Provini and Patrizia Teofoli and Debora Pomponi and Ornella de Pit{\`a}},
  journal={Allergy},
  year={2006},
  volume={61 9},
  pages={1071-7}
}
BACKGROUND Functional autoantibodies against the alpha-chain of the high-affinity IgE receptor (FcepsilonRIalpha) identify a subset of patients with chronic urticaria (CU) due to autoreactivity, as assessed by an in vivo positive response to autologous serum skin test (ASST). We performed a study to standardize the serum-induced basophil activation assay by flow cytometry (FCM) using a new tricolour method, assessing the diagnostic performance of this test in discriminating between ASST+ and… CONTINUE READING
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