Serum-free transfection of CHO cells with chemically defined transfection systems and investigation of their potential for transient and stable transfection.

Abstract

The generation of transgenic cell lines is acquired by facilitating the uptake and integration of DNA. Unfortunately, most of the systems generating stable expression systems are cost and time-consuming and transient expression is optimized to generate milligram amounts of the recombinant protein. Therefore we improved and compared two transfection systems… (More)
DOI: 10.1007/s10616-009-9224-x

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