Serum-free media in hybridoma culture and monoclonal antibody production.

  title={Serum-free media in hybridoma culture and monoclonal antibody production.},
  author={Mark C. Glassy and Joe Tharakan and P. C. Chau},
  journal={Biotechnology and bioengineering},
  volume={32 8},
The replacement of serum in hybridoma cultures is considered. The focus is on the effects of serum-free media on hybridoma growth and monoclonal antibody secretion. Comparative literature data with serum supplemented cultures are discussed with an analysis of serum-free formulations and selection rules for the serum-free ingredients. In general, serum-free media which are "lipid rich" can sustain cell growth rates approaching that of serum supplemented cultures. Specific antibody secretion rate… CONTINUE READING

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