Serum-free culture and characterization of renal epithelial cells isolated from human fetal kidneys of varying gestational age

Abstract

Cell cultures were initiated from seven human fetal kidneys that varied in gestational age from 90 days to newborn. The growth medium utilized was a 1:1 mixture of Dulbecco’s modified Eagle’s and Ham’s F12 supplemented with selenium (5 ng/ml), insulin (5µg/ml), transferrin (5µg/ml), hydrocortisone (36 ng/ml), triiodothyronine (4 pg/ml), and epidermal growth… (More)
DOI: 10.1007/BF02634355

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@article{HazenMartin1994SerumfreeCA, title={Serum-free culture and characterization of renal epithelial cells isolated from human fetal kidneys of varying gestational age}, author={Debra J. Hazen-Martin and Betty Tarnowski and John H. Todd and Mary Ann Sens and John E. Bylander and Brendan Smyth and Alexander J Garvin and Donald A Sens}, journal={In Vitro Cellular & Developmental Biology - Animal}, year={1994}, volume={30}, pages={356-365} }