Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent.

Abstract

Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.

01002003002008200920102011201220132014201520162017
Citations per Year

952 Citations

Semantic Scholar estimates that this publication has 952 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Molina2008SerumderivedHC, title={Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent.}, author={Sonia Molina and Val{\'e}rie Castet and Lydiane Pichard-Garcia and Czeslaw Wychowski and Eliane F Meurs and Jean Marc Pascussi and Camille Sureau and J Fabre and Antonio Sacunha and Dominique G Larrey and Jean Dubuisson and Joliette Coste and Jane A McKeating and Patrick Maurel and Chantal Fournier-Wirth}, journal={Journal of virology}, year={2008}, volume={82 1}, pages={569-74} }