Serum cytotoxicity to pig cells and anti-alphaGal antibody level and specificity in humans and baboons.

@article{Neethling1999SerumCT,
  title={Serum cytotoxicity to pig cells and anti-alphaGal antibody level and specificity in humans and baboons.},
  author={Francisca A Neethling and David K C Cooper},
  journal={Transplantation},
  year={1999},
  volume={67 5},
  pages={658-65}
}
BACKGROUND Removal and/or "neutralization" of anti-Gal alpha1-3Gal (alphaGal) antibodies can prevent or delay the hyperacute rejection of pig organs transplanted into primates. AIM To determine variations in (1) cytotoxicity to pig kidney (PK15) cells, (2) anti-alphaGal antibody level, and (3) specificity in adult human (n=46) and baboon (n=38) sera. METHODS Cytotoxicity to PK15 cells was determined by adding rabbit complement to heat-inactivated serum, using a two-color fluorescent dye to… CONTINUE READING