Serum apolipoprotein A-1 quantification by LC–MS with a SILAC internal standard reveals reduced levels in smokers

@inproceedings{Wang2015SerumAA,
  title={Serum apolipoprotein A-1 quantification by LC–MS with a SILAC internal standard reveals reduced levels in smokers},
  author={Qingqing Wang and Suhong Zhang and Lili Guo and Christine M. Busch and Wenying Jian and Naidong Weng and Nathaniel W. Snyder and Kannan Rangiah and Clementina A. Mesaros and Ian A Blair},
  booktitle={Bioanalysis},
  year={2015}
}
BACKGROUND Absolute quantification of protein biomarkers such as serum apolipoprotein A1 by both immunoassays and LC-MS can provide misleading results. RESULTS Recombinant ApoA-1 internal standard was prepared using stable isotope labeling by amino acids in cell culture with [(13)C6(15)N2]-lysine and [(13)C9(15)N1]-tyrosine in human cells. A stable isotope dilution LC-MS method for serum ApoA-1 was validated and levels analyzed for 50 nonsmokers and 50 smokers. CONCLUSION The concentration… CONTINUE READING

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