Serum alkaline phosphatase: total activity and isoenzyme determinations made by use of the centrifugal fast analyzer.

@article{Statland1972SerumAP,
  title={Serum alkaline phosphatase: total activity and isoenzyme determinations made by use of the centrifugal fast analyzer.},
  author={Bernard E. Statland and Hiroyuki Nishi and Donald S. Young},
  journal={Clinical chemistry},
  year={1972},
  volume={18 12},
  pages={1468-74}
}
We present a kinetic method in which the centrifugal analyzer is used to determine total alkaline phosphatase activity and to measure the isoenzymes in bone, liver, and the L-phenylalanine-sensitive fraction (intestine, tumor, and placenta). Measurements were made at 30#{176}C in diethanolamine buffer, with p-nitrophenylphosphate as substrate. The a!kaline phosphatase isoenzymes were separated and measured by selective chemical inhibition with 10 mmol/liter Lphenylalanine and 3.3 mol urea per… CONTINUE READING
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