Sertoli and Leydig cells of the human testis express neurofilament triplet proteins

@article{Davidoff1999SertoliAL,
  title={Sertoli and Leydig cells of the human testis express neurofilament triplet proteins},
  author={Michail S. Davidoff and Ralf Middendorff and Wolfgang Pusch and Dieter M{\"u}ller and S. Wichers and Adolf F. Holstein},
  journal={Histochemistry and Cell Biology},
  year={1999},
  volume={111},
  pages={173-187}
}
 Using RT-PCR, western blot and enzyme and fluorescence immunocytochemical techniques, the three isoforms of neurofilament proteins (NFPs), namely NF-L (NFP-68 kDa), NF-M (NFP-160 kDa) and NF-H (NFP-200 kDa) were found in Sertoli and Leydig cells of human testes. RT-PCR showed specific for the three NFP fragments in testicular tissue, in isolated seminiferous tubules and in isolated Leydig cells. In protein preparations from the same testicular components, western blot analysis detected bands… CONTINUE READING

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Dual nature of Leydig cells of the human testis

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