Serotypes of bovine Escherichia coli producing cytotoxic necrotizing factor type 2 (CNF2).

  title={Serotypes of bovine Escherichia coli producing cytotoxic necrotizing factor type 2 (CNF2).},
  author={M. Blanco and J. Blanco and A. Verbruggen and W. Jansen},
  journal={Veterinary microbiology},
  volume={39 1-2},
The serotypes of 101 faecal bovine necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factor type 2 (CNF2) were determined. Although, NTEC producing CNF2 belonged to 48 different O:K:H serotypes, only eleven of them accounted for 54% of strains. The most common serotypes in order of frequency were: O123:K-:H16, O3:K-:H21, O88:K-:H8, O15:K14:H21, O1:K-:H12, O1:K1:H46, O2:K1:H5, O55:H21, O88:K?:H25, O117:K?:H21 and O123:K-:H-. The serotypes of CNF2 NTEC were different from… Expand
Serotypes of CNF1-producingEscherichia coli strains that cause extraintestinal infections in humans
The results show that extraintestinal infections are caused by a limited number of virulent clones, as suggested by the theory of special pathogenicity. Expand
Distribution and characterization of faecal necrotoxigenic Escherichia coli CNF1+ and CNF2+ isolated from healthy cows and calves.
Faecal swabs obtained from a random sample of 268 cows and 90 calves on 19 Lugo (northwestern Spain) farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizingExpand
Prevalence and characteristics of necrotoxigenic Escherichia coli CNF1+ and CNF2+ in healthy cattle.
It is confirmed that healthy cattle are a reservoir of NTEC producing C NF2, and revealed that CNF2+ strains are more frequently carried by calves than by adult cows. Expand
Serogroups, toxins and antibiotic resistance of Escherichia coli strains isolated form diarrhoeic goat kids in Spain.
It is concluded that E. coli strains isolated from diarrhoeic goat kids are usually non-toxigenic and belong to a large number of serogroups, and the highest percentages of antibiotic resistance were streptomycin, sulfadiazine, kanamycin, neomycin and ampicillin. Expand
Necrotoxigenic Escherichia coli from sheep and goats produce a new type of cytotoxic necrotizing factor (CNF3) associated with the eae and ehxA genes.
The association of cnf3 with eae and ehxA suggests that cnF3+ NTEC strains might be pathogenic for humans. Expand
Prevalence and characteristics of necrotoxigenic Escherichia coli (NTEC) strains isolated from diarrhoeic dairy calves.
A strong association between CNF2 and F17 fimbriae was found and a significant age-associated increase in the prevalence of NTEC producing C NF2 (p<0.0001) was found. Expand
CNF producing Escherichia coli isolated from cattle in Northern Ireland.
Serogrouping of CNF isolates did not reveal the association of any particular serogroups with the different conditions, and tissue culture assays used to investigate the incidence of cytotoxic necrotising factors 1 and 2 in Escherichia coli strains from cattle found no association. Expand
Polymerase chain reaction for detection of Escherichia coli strains producing cytotoxic necrotizing factor type 1 and type 2 (CNF1 and CNF2)
The PCR protocol describe here permits rapid and accurate detection of CNF1 and CNF2 genes in Escherichia coli strains isolated from clinical material and contaminated foods. Expand
DNA Hybridization of Escherichia coli Strains Isolated from Uteri and Fecal Samples of Bitches with Pyometra
Findings suggest that pathogenic E. coli may play an important role in pyometra and the strains isolated from the uteri may be similar to isolates from feces. Expand


Serogroups of Escherichia coli strains producing cytotoxic necrotizing factors CNF1 and CNF2.
The serogroups of 396 necrotizing Escherichia coli of human and bovine origin isolated in Spain between 1979 and 1991 have been determined and it is found that the majority of necrotized E. coli producing cytotoxic necrotizer factor 1 strains were obtained from human extraintestinal infections, whereas cytot toxic necrotization factor 2 strains were isolated from stools of healthy and diarrhoeic calves. Expand
Virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of Escherichia coli
This study confirmed that C NF2-producing strains are unrelated to previously described toxigenic E. coli strains and demonstrated that in half of the strains the production of CNF2 was associated with an adhesion factor genetically related to, but different from, F17, which is more than likely encoded by Vir plasmids. Expand
Serotypes and antibiotic resistance of verotoxigenic (VTEC) and necrotizing (NTEC) Escherichia coli strains isolated from calves with diarrhoea.
VTEC were resistant to antibiotics at higher rates than NTEC and non-VTEC non-NTEC, and showed also the highest multidrug-resistant pattern. Expand
Relationship between cytotoxic necrotizing factor production and serotype in hemolytic Escherichia coli
Serological typing confirmed that hemolytic isolates of E. coli may be divided into two main populations on the basis of the ability to produce CNF, and was significantly more prevalent among strains from extraintestinal infections than among those from stools. Expand
Production of toxins by Escherichia coli strains isolated from calves with diarrhoea in galicia (north-western Spain).
The results indicate that VT-producing and CNF-producing E. coli strains are frequently isolated from diarrhoeic calves and that according to the serotypes exhibited, some of them might be considered potential pathogens for humans. Expand
Cytotoxic necrotizing factor production by hemolytic strains of Escherichia coli causing extraintestinal infections
Two hundred and nineteen strains of Escherichia coli from extraintestinal infections and feces of healthy subjects were examined for hemolysin and cytotoxic necrotizing factor production and for mannose-resistant hemagglutination, suggesting the existence of two distinct classes among hemolytic strain E. coli. Expand
Gene block encoding production of cytotoxic necrotizing factor 1 and hemolysin in Escherichia coli isolates from extraintestinal infections
DNA hybridization experiments indicated that the CNF1 and hemolysin determinants were closely linked on the chromosomes of isolate E-B35 and six additional extraintestinal isolates belonging to serogroups O2, O4, O6, O22, O75, and O85. Expand
Virulence factors of bacteraemic Escherichia coli with particular reference to production of cytotoxic necrotising factor (CNF) by P-fimbriate strains.
Virulence factors were strongly associated with strains expressing defined MRHA types, especially in strains of O4 and O6 groups, and all 12 P-fimbriate strains expressed the MRHA type IVa and 11 of them synthesised CNF. Expand
Cytotoxins in non-enterotoxigenic strains of Escherichia coli isolated from feces of diarrheic calves.
Seroneutralization assays showed that cytotoxins of the same type were closely related antigenically and a slight cross-neutralization was observed between Type 1 (CNF) and Type 2 (Vir) toxins. Expand
Frequent production of toxins by Escherichia coli strains isolated from human urinary tract infections: relation with haemagglutination
It is concluded that, in addition to haemolytic and haemagglutinating activities, the production of CNF was closely associated to virulence in E. coli strains isolated from urinary tract infections. Expand