Serotype-specific replicating AAV helper constructs increase recombinant AAV type 2 vector production.

@article{Li2005SerotypespecificRA,
  title={Serotype-specific replicating AAV helper constructs increase recombinant AAV type 2 vector production.},
  author={Chengwen Li and Richard Jude Samulski},
  journal={Virology},
  year={2005},
  volume={335 1},
  pages={10-21}
}
One of the major limitations of the use of adeno-associated virus (AAV) as a tool for gene therapy is the difficulty in providing sufficient quantities of the virus for pre-clinical and clinical trials. Here, we report a novel approach for amplifying AAV helper functions, which mimics the normal replication of wild type (wt) AAV resulting in a high yield of… CONTINUE READING