Serotype identification of avian infectious bronchitis virus by RT-PCR of the peplomer (S-1) gene.

@article{Keeler1998SerotypeIO,
  title={Serotype identification of avian infectious bronchitis virus by RT-PCR of the peplomer (S-1) gene.},
  author={Calvin L. Keeler and Karen L. Reed and Wilfried Nix and Jack Gelb},
  journal={Avian diseases},
  year={1998},
  volume={42 2},
  pages={275-84}
}
The S-1 peplomer gene sequences of 31 strains of avian coronavirus infectious bronchitis virus (IBV) from North America, Europe, and Australia were compared to identify common and unique regions for possible diagnostic applications. S-1 sequences that were conserved among serotypes and sequences that were variable between serotypes were identified. Based on conserved S-1 gene sequences, "general" degenerate oligonucleotide primers were designed that amplified IBV genomic RNA by the reverse… CONTINUE READING