Serotonin stimulates mitogen-activated protein kinase activity through the formation of superoxide anion.

@article{Lee1999SerotoninSM,
  title={Serotonin stimulates mitogen-activated protein kinase activity through the formation of superoxide anion.},
  author={Sheu-Ling Janet Lee and Wei-wei Wang and Geraldine A. Finlay and Barry L Fanburg},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={1999},
  volume={277 2},
  pages={L282-L291}
}
  • S. Lee, Wei-wei Wang, +1 author B. Fanburg
  • Published 1999
  • Biology, Medicine
  • American journal of physiology. Lung cellular and molecular physiology
Our previous studies have shown that, through an active transport process, serotonin (5-HT) rapidly elevates[Formula: see text] formation, stimulates protein phosphorylation, and enhances proliferation of bovine pulmonary artery smooth muscle cells (SMCs). We presently show that 1 μM 5-HT also rapidly elevates phosphorylation and activation of the mitogen-activated protein (MAP) kinases extracellular signal-regulated kinase (ERK) 1 and ERK2 of SMCs, and the enhanced phosphorylation is blocked… Expand
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Phospholipase D signaling in serotonin-induced mitogenesis of pulmonary artery smooth muscle cells.
  • Y. Liu, B. Fanburg
  • Biology, Medicine
  • American journal of physiology. Lung cellular and molecular physiology
  • 2008
TLDR
Results indicate that ligation of the 5-HTR 2A by 5-HT initiates PLD activation in SMCs, and that its product, PA, is an early signaling molecule in 5- HT-induced pulmonary artery SMC proliferation. Expand
Inability of Serotonin to Activate the c-Jun N-terminal Kinase and p38 Kinase Pathways in Rat Aortic Vascular Smooth Muscle Cells
TLDR
The conclusion that 5-HT does not activate the JNK or p38 pathways in rat vascular smooth muscle cells is supported. Expand
Rho Kinase–Induced Nuclear Translocation of ERK1/ERK2 in Smooth Muscle Cell Mitogenesis Caused by Serotonin
TLDR
The studies show for the first time to the knowledge combinational action of SERT and a 5-HT receptor in SMC growth and Rho A/ROCK participation in 5- HT receptor 1B/1D-mediated mitogenesis of vascular SMCs through an effect on cytoplasmic to nuclear translocation of ERK1/ERK2. Expand
5-HT Induction of c-fos gene expression requires reactive oxygen species and rac1 and ras GTPases
TLDR
It is shown by Western blot that 5-HT upregulates c-Fos, an immediate early gene product known to regulate the entrance of quiescent cells into the cell cycle, and Regulation of cyclin D1, a protein shown to be regulated by c-fos and required for entry into thecell cycle, is upregulated by 5- HT and is blocked by DPI and PD98059. Expand
Activation of Erk Mitogen-Activated Protein Kinase Proteins by Vascular Serotonin Receptors
TLDR
While mRNA and possibly protein for multiple 5-HT receptors are present in aortic smooth muscle, only the 5- HT 2A receptor plays a significant role in directly modulating contractility and activating the Erk MAPK pathway. Expand
H(2)O(2) signals 5-HT-induced ERK MAP kinase activation and mitogenesis of smooth muscle cells.
TLDR
Generation of O(2)(-) in BPASMCs in response to 5-HT is followed by an increase in intracellular H( 2)O(2) that mediates 5- HT-induced mitogenesis through activation of ERK1/ERK2 but not of p38 MAP kinase. Expand
Inhibition of serotonin-induced mitogenesis, migration, and ERK MAPK nuclear translocation in vascular smooth muscle cells by atorvastatin.
TLDR
It is suggested that atorvastatin inhibits 5-HT-induced PASM cell mitogenesis and migration through targeting isoprenylation which may, in part, attenuate the Rho pathway, a mechanism that may apply to statin effects on in vivo models of pulmonary hypertension. Expand
JNK regulates serotonin-mediated proliferation and migration of pulmonary artery smooth muscle cells.
TLDR
It is demonstrated that JNK activity is necessary for serotonin (5-HT)-induced proliferation and migration of bovine pulmonary artery smooth muscle cells (PASMCs) and may act at an important point for cross talk of the MAPK and PI3K pathways. Expand
5-HT 2 A receptors stimulate mitogen-activated protein kinase via H 2 O 2 generation in rat renal mesangial cells
Greene, Eddie L., Odette Houghton, Georgiann Collinsworth, Maria N. Garnovskaya, Toshio Nagai, Tahir Sajjad, Venugopala Bheemanathini, Jasjit S. Grewal, Richard V. Paul, and John R. Raymond. 5-HT2A… Expand
A new hypertrophic mechanism of serotonin in cardiac myocytes: receptor‐independent ROS generation
TLDR
The data suggest that part of cardiac hypertrophic effect of serotonin requires hydrogen peroxide production by MAO A and ERK1/2 activation, and this newly recognized, receptor‐independent mechanism of serotonin may contribute to myocardial remodeling and failure. Expand
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References

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TLDR
The present studies demonstrate rapid enhancement by 5-HT of Tyr phosphorylation of proteins, including p120, which also occurs in SMC but not in EC, and indicate that Tyr phosphORYlation of GAP may act as an intermediate signal in5-HT-induced mitogenesis of SMC which requires cellular internalization of 5- HT rather than its action on a membrane receptor. Expand
Superoxide as an intermediate signal for serotonin-induced mitogenesis.
TLDR
It is concluded that 5-HT induced cellular proliferation of SMC through signaling pathways that utilize its transport system and O2.- formation. Expand
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TLDR
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TLDR
It is demonstrated that tyrosine kinase activation may partially mediate contractility to 5-HT in arterial smooth muscle, and tyrphostin 23 is somewhat nonselective and5-HT stimulates tyrosin kinase as documented by increased tyrosyl phosphorylation of proteins in cultured aortic smooth muscle cells and aorti tissue in active contraction of 5- HT. Expand
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Vascular 5-Hydroxytryptamine2A (5-HT2A) receptor signaling and contraction has been associated with the activation of L-type calcium channels, phospholipase C (PLC) and, as we previously… Expand
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