Seroreactivity to and avidity for recombinant antigens in toxoplasmosis.

  title={Seroreactivity to and avidity for recombinant antigens in toxoplasmosis.},
  author={K Pfrepper and Gisela Enders and Marion Gohl and Doris Krczal and Harald Hlobil and Doris Wassenberg and Erwin Soutschek},
  journal={Clinical and diagnostic laboratory immunology},
  volume={12 8},
To improve serodiagnostic methods for the diagnosis of acute toxoplasmosis during pregnancy, a new test system has been developed and evaluated based on the use of recombinant antigens. Five recombinant Toxoplasma gondii antigens (ROP1, MAG1, SAG1, GRA7, and GRA8) were cloned in Escherichia coli, purified, and applied directly onto nitrocellulose membranes in a line assay (recomLine Toxoplasma). A panel of 102 sera from 25 pregnant women with supposed recent toxoplasmosis and from two… CONTINUE READING