Serological detection of grapevine virus a using antiserum to a nonstructural protein, the putative movement protein.

@article{Rubinson1997SerologicalDO,
  title={Serological detection of grapevine virus a using antiserum to a nonstructural protein, the putative movement protein.},
  author={Eileen Rubinson and Nurbol Galiakparov and Serban Radian and Ilan Sela and Edna Tanne and Ron Gafny},
  journal={Phytopathology},
  year={1997},
  volume={87 10},
  pages={1041-5}
}
ABSTRACT Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation… CONTINUE READING