Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS.

@article{Schpbach1984SerologicalAO,
  title={Serological analysis of a subgroup of human T-lymphotropic retroviruses (HTLV-III) associated with AIDS.},
  author={J{\"o}rg Sch{\"u}pbach and Mikul{\'a}{\vs} Popovi{\vc} and Raymond V. Gilden and Matthew A. Gonda and M. G. Sarngadharan and Robert C. Gallo},
  journal={Science},
  year={1984},
  volume={224 4648},
  pages={
          503-5
        }
}
The two main subgroups of the family of human T-lymphotropic retroviruses (HTLV) that have previously been characterized are known as HTLV-I and HTLV-II. Both are associated with certain human leukemias and lymphomas. Cell surface antigens (p61 and p65) encoded by HTLV-I are frequently recognized, at low titers, by antibodies in the serum of patients with acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that precede AIDS (pre-AIDS). This suggests an involvement of HTLV in… 
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Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS.
TLDR
Serum samples from 88 percent of patients with AIDS and from 79 percent of homosexual men with signs and symptoms that frequently precede AIDS, but from less than 1 percent of heterosexual subjects, have antibodies reactive against antigens of HTLV-III, and the major immune reactivity appears to be directed against p41, the presumed envelope antigen of the virus.
An HTLV-III peptide produced by recombinant DNA is immunoreactive with sera from patients with AIDS
TLDR
The production, by genetic engineering methods, of a peptide of relative molecular mass 15,000 which was strongly immunoreactive with anti-HTLV-III antibodies present in sera from AIDS patients is suggested, suggesting that the peptide may be useful for detecting anti- HTLV- III antibodies in blood samples.
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell…
Serologic identification and characterization of a macaque T-lymphotropic retrovirus closely related to HTLV-III.
TLDR
The serologic characterization of a new simian retrovirus that is related to HTLV-III is described, which naturally infects a nonhuman primate species and may provide a useful model for the study of HT LV-III and the pathogenesis of AIDS.
Serological characterization of HTLV-III infection in AIDS and related disorders.
TLDR
There was no apparent difference in pattern of antibodies to HTLV-III-related antigens in symptomatic vs. asymptomatic seropositive individuals and a short incubation time in the immunoblot assay failed to detect low-titer or low-affinity antibodies that were detected by overnight incubation.
Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients
TLDR
It is reported here that sera from subjects in various disease categories possess only low-level neutralizing activity, even when antibodies to viral membrane antigens are present in high titre.
A pathogenic retrovirus (HTLV-III) linked to AIDS.
TLDR
There are new possibilities for identifying early cases of AIDS through antibody testing, and the discovery of an immunosuppressive retrovirus in AIDS could serve as a stimulus to re-examine endemic forms of cancer in Third World countries.
Antibodies to HTLV-III in Swiss patients with AIDS and pre-AIDS and in groups at risk for AIDS.
TLDR
Antibodies to the major viral protein p24 were found consistently and at high titers in the seropositive members of the groups at risk and in those with pre-AIDS but were dramatically reduced in patients with AIDS, while the ELISA test that is currently available is a useful screening tool.
Molecular cloning and characterization of the HTLV-III virus associated with AIDS
TLDR
The availability of the cloned HTLV-III genome will now allow an unambiguous comparison of this virus with other retroviruses that also have been associated with the pathogenesis of AIDS8–11, and will facilitate the development of diagnostic and therapeutic measures in the treatment of AIDS.
Human T-cell lymphotropic virus type III: immunologic characterization and primary structure analysis of the major internal protein, p24
TLDR
The major internal structural protein of human T-cell lymphotropic virus type III (HTLV-III), a virus etiologically implicated in acquired immunodeficiency syndrome (AIDS), was purified to homogeneity and shown to lack immunologic cross-reacting antigenic determinants shared by other known retroviruses.
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References

SHOWING 1-10 OF 51 REFERENCES
Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS.
TLDR
Serum samples from 88 percent of patients with AIDS and from 79 percent of homosexual men with signs and symptoms that frequently precede AIDS, but from less than 1 percent of heterosexual subjects, have antibodies reactive against antigens of HTLV-III, and the major immune reactivity appears to be directed against p41, the presumed envelope antigen of the virus.
Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS.
Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell…
Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS).
TLDR
From these studies it is concluded that this virus as well as the previous HTLV isolates belong to a general family of T-lymphotropic retroviruses that are horizontally transmitted in humans and may be involved in several pathological syndromes, including AIDS.
Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS.
TLDR
A cell system was developed for the reproducible detection of human T-lymphotropic retroviruses (HTLV family) from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS), and it provides large amounts of virus for detailed molecular and immunological analyses.
Isolation of human T-cell leukemia virus in acquired immune deficiency syndrome (AIDS).
TLDR
Whether or not HTLV-I or other retroviruses of this family with T-cell tropism cause AIDS, it is possible that patients from whom the virus can be isolated can also transmit it to others.
Proviral DNA of a retrovirus, human T-cell leukemia virus, in two patients with AIDS.
TLDR
In an attempt to find evidence of HTLV infection in patients with AIDS, DNA from samples of peripheral blood lymphocytes from 33 AIDS patients was analyzed by Southern blot-hybridization with a radiolabeled cloned HTLV DNA probe and the presence of integrated HTLV proviral sequences in lymphocytes was revealed.
A new subtype of human T-cell leukemia virus (HTLV-II) associated with a T-cell variant of hairy cell leukemia.
TLDR
Human T-cell leukemia virus (HTLV) is a human type-C RNA tumor virus (retrovirus) previously identified in and isolated from several patients with T- cell leukemias or lymphomas, but a related retrovirus has been found in a patient with a somewhat different disease.
Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus
TLDR
It is found that antibodies against p24 are present in human sera (including those of patient C.R. and his wife), and that these are specifically directed at HTLVCR proteins and not at cell-specific determinants—the first evidence for a specific immune response in humans against a retrovirus.
Antibodies against three purified structural proteins of the human type‐C retrovirus, HTLV, in Japanese adult T‐cell leukemia patients, healthy family members, and unrelated normals
TLDR
Results provide strong and detailed immunological evidence that the human retrovirus isolates first made from US patients with cutaneous T‐cell malignancies, and those made later in Japanese ATL are either identical or very closely related strains of the same virus, HTLV, a finding verified in other detailed analyses of the HTLV genomes of the respective isolates.
Transformation and cytopathogenic effect in an immune human T-cell clone infected by HTLV-I.
TLDR
In the present experiments, T cells specifically reactive against HTLV were propagated from a patient whose HTLV-bearing lymphoma was in remission and an HT LV-specific cytotoxic T-cell clone was isolated.
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