Serodiagnosis of melioidosis in Malaysia.

  title={Serodiagnosis of melioidosis in Malaysia.},
  author={Jamuna Vadivelu and Savithri Devi Puthucheary and Gurmil S. Gendeh and Navaratnam Parasakthi},
  journal={Singapore medical journal},
  volume={36 3},
Current diagnosis of melioidosis is based on bacterial culture and/or serology which is becoming increasingly useful. An IgM-ELISA using heat-killed whole cells of Pseudomonas pseudomallei was developed and compared with an indirect haemagglutination technique (IHAT) and an indirect immunofluorescent technique(IFAT). The IgM-ELISA using a P:N ratio of > or = 2 had a sensitivity of 91% and a specificity of 96%. All 3 assays were further used in a seroepidemiological survey amongst different… CONTINUE READING
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