Serodiagnosis of cryptosporidiosis in Italian HIV-positive patients by means of an oocyst soluble antigen in an ELISA.

@article{Morales1992SerodiagnosisOC,
  title={Serodiagnosis of cryptosporidiosis in Italian HIV-positive patients by means of an oocyst soluble antigen in an ELISA.},
  author={M A Gomez Morales and E Pozio and G P Croppo},
  journal={The Journal of infection},
  year={1992},
  volume={25 2},
  pages={229-36}
}
A sensitive, specific, and reproducible ELISA which incorporated an oocyst soluble antigen was used in order to detect specific Cryptosporidium immunoglobulins G and M in Italian HIV-positive patients. The soluble antigen was prepared from purified oocysts collected from experimentally infected calves. The serum working dilution was established at 1 in 50. Of parasitologically positive and HIV-positive patients, 95% showed specific IgG or IgM or both in their serum. Of HIV-positive patients… CONTINUE READING