Serine proteases activity is important for the interaction of nematophagous fungus Duddingtonia flagrans with infective larvae of trichostrongylides and free-living nematodes Panagrellus spp.

  title={Serine proteases activity is important for the interaction of nematophagous fungus Duddingtonia flagrans with infective larvae of trichostrongylides and free-living nematodes Panagrellus spp.},
  author={D. Cruz and Luana M Costa and L. O. Rocha and C. Retamal and R. Vieira and S. Seabra and C. Silva and R. DaMatta and C. P. Santos},
  journal={Fungal biology},
  volume={119 8},
The nematode-trapping fungus Duddingtonia flagrans has been studied as a possible control method for gastrointestinal nematodes of livestock animals. These fungi capture and infect the nematode by cuticle penetration, immobilization, and digestion of the internal contents. It has been suggested that this sequence of events occurs by a combination of physical and enzymatical activities. The aim of this study was to investigate the participation of proteolytic enzymatic activity during the… Expand
iTRAQ-based quantitative proteomic analysis of mycelium in different predation periods in nematode trapping fungus Duddingtonia flagrans
These findings not only deepen the understanding of the proteome during different predation periods in Duddingtonia flagrans but also provide a basis for screening and identification of predation-related proteins to reveal the biological processes of the fungus and its action on nematodes. Expand
The potential of plant and fungal proteins in the control of gastrointestinal nematodes from animals.
This work discusses the use of exogenous proteins and peptides in the control of gastrointestinal nematode infection with a focus on animal nematodes. Expand
Morphological and molecular characterization, predatory behaviour and effect of organic extracts of four nematophagous fungi from Mexico
Four nematophagous fungi were identified by traditional and molecular techniques, and their in vitro predatory activity (PA) against Haemonchus contortus (Hc), and Panagrellus redivivus (Pr) was assessed, and they are promising candidates as potential biocide agents against parasitic nematodes. Expand
RNA-Seq reveals the molecular mechanism of trapping and killing of root-knot nematodes by nematode-trapping fungi
The findings illustrate the molecular mechanism of fungal parasitism in A. conoides which may be helpful in developing a potential biocontrol agent against parasitic nematodes. Expand
Advances in pathogenicity-related serine proteases in nematophagous fungi
Foundation item: National Natural Science Foundation of China (No. U1402265, 31272093); Program for Excellent Young Talents of Yunnan University *Corresponding author: Tel: 86-871-65032538; E-mail:Expand
Esta revisao tem o objetivo of abordar os principais helmintos em equinos e suas formas de controle, ressaltando o potencial of D. flagrans como alternativa no controle biologico dos parasitos. Expand
Duddingtonia flagrans preying a plant parasitic nematode.


Acid phosphatase activity during the interaction of the nematophagous fungus Duddingtonia flagrans with the nematode Panagrellus sp.
The participation of acid phosphatase activity and its importance during the interaction of the fungus with the nematode were discussed and this enzyme was significantly higher for the fungus-nematode interaction when compared to the organisms alone, indicating a synergistic response. Expand
An extracellular serine protease of an isolate of Duddingtonia flagrans nematophagous fungus
A serine protease produced by D. flagrans (AC001) has been isolated, which is effective in the in vitro destruction of cyathostomin L3. Expand
Proteases and Their Involvement in the Infection and Immobilization of Nematodes by the Nematophagous Fungus Arthrobotrys oligospora
The effects of PMSF on the immobilization of nematodes were probably due to serine proteases produced by the fungus, since the effects were observed when unbound inhibitor was washed away from the fungus before the nematode was added to the system. Expand
Pre-penetration events in fungal parasitism of nematode eggs
Hydrophobicity, appresorium formation and protease production as factors involved in early parasitism of nematode eggs are shown. Expand
Predatory behaviour of trapping fungi against srf mutants of Caenorhabditis elegans and different plant and animal parasitic nematodes.
A bioassay was designed to investigate the predatory response of several isolates of nematode-trapping fungi against 3 mutants of Caenorhabditis elegans, which have been reported to differ in the reaction of their cuticle to antibodies and lectins. Expand
Purification and cloning of a novel serine protease from the nematode-trapping fungus Dactylellina varietas and its potential roles in infection against nematodes
A novel pathogenic protease from the nematode-trapping fungus D. varietas is identified and the three-dimensional structure of this protease was predicted using the Swiss-Prot method, suggesting it may play a role in infection against nematodes. Expand
Nematophagous fungi for biological control of gastrointestinal nematodes in domestic animals
Nematophagous fungi in general are ‘old friends’ that are ready to the ‘fight with the authors' old enemies’, the gastrointestinal helminth parasites harmful to human and animal health. Expand
Extracellular enzymes and the pathogenesis of nematophagous fungi
The roles of extracellular hydrolytic enzymes and other virulence factors involved in infection against nematodes were summarized and the biochemical properties and peptide sequences of a special group of enzymes were compared, and their implications in infections were discussed. Expand
Characterization of an extracellular protease and its cDNA from the nematode-trapping fungus Monacrosporium microscaphoides.
This report is among the first to describe the purification and cloning of an infectious protease from a different nematode-trapping fungus, suggesting that Mlx could serve as a virulence factor during infection. Expand
Improving the Pathogenicity of a Nematode-Trapping Fungus by Genetic Engineering of a Subtilisin with Nematotoxic Activity
It is reported that a subtilisin-like extracellular serine protease designated PII is an important pathogenicity factor in the common nematode-trapping fungus Arthrobotrys oligospora and the paralyzing activity of PII was verified by demonstrating that a heterologous-produced PII had a nematotoxic activity when added to free-living nematodes. Expand