Serine protease from midgut of Bombus terrestris males.

  title={Serine protease from midgut of Bombus terrestris males.},
  author={Jana Brabcov{\'a} and Jiř{\'i} Kindl and Irena Valterov{\'a} and Iva Pichov{\'a} and Marie Zarev{\'u}ck{\'a} and Jana Brabcov{\'a} and Michal J{\'a}gr and Ivan Mik{\vs}{\'i}k},
  journal={Archives of insect biochemistry and physiology},
  volume={82 3},
A serine protease was isolated from midguts of the bumblebee male Bombus terrestris by a combination of precipitation procedures with column chromatography. The purified enzyme exhibited two bands with molecular masses of 25 and 26 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. These bands showed a proteolytic activity in zymography assay. Midgut enzymes showed optimum proteolytic activity at pH 9 and 35°C using N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenyl… Expand
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