Serine/threonine protein phosphatase assays.

@article{McAvoy2010SerinethreoninePP,
  title={Serine/threonine protein phosphatase assays.},
  author={Thomas McAvoy and Angus C. Nairn},
  journal={Current protocols in molecular biology},
  year={2010},
  volume={Chapter 18},
  pages={
          Unit18.18
        }
}
Methods for assaying serine/threonine protein phosphatases are discussed. Three commonly used protocols are presented that employ either colorimetric or radiometric assays. These methods can be used to assay a variety of preparations of serine/threonine phosphatases, from crude lysates to purified proteins. Strategies for the application of a particular protocol for a particular purpose are discussed. The assays can be used in either a high-throughput mode where simple comparison of activities… CONTINUE READING
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