Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus.

Abstract

The Cowden strain of porcine group C rotavirus (pararotavirus) was adapted to serial passage in a continuous monkey kidney cell line (MA104). Key factors in its successful adaptation included use of virus passaged in primary porcine kidney cells as the initial inoculum, use of roller tubes, and addition of pancreatin to the maintenance medium. A cell… (More)

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