Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle

  title={Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle},
  author={David Yaffe and O Saxel},
THE muscular dystrophies are a group of hereditary disorders manifested by a progressive wasting of the skeletal muscles. In spite of extensive studies, the nature of the primary lesion is unknown (for review see ref. 1). Because of the complex interaction between tissues, it is difficult to study this question in vivo. Therefore attempts have been made to investigate this question in cultures of dystrophic muscles of human or animal origin. Tissue explants as well as monolayer primary cell… Expand
A muscle cell line from dystrophic mice expressing an altered phenotype in vitro.
Because they can be grown in large amounts, and represent a pure muscle cell population which express an altered phenotype in an in vitro aneural avascular environment, DyA4 cells provide a very useful model system for investigating the pathogenesis of murine muscular dystrophy. Expand
Tissue culture studies of muscle disorders: Part 2. Biochemical studies, nerve‐muscle culture, metabolic myopathies, and animal models
Study of protein, lipid, and purine metabolism of Duchenne muscular dystrophy cells in vitro and of muscle cells in combined culture with nerve cells find suggestions of altered collagen synthesis by DMD cells. Expand
Skeletal muscle cultures.
Publisher Summary This chapter reviews the preparation of primary muscle cell cultures, and the characteristics of different established muscle cell lines and the special culture conditions andExpand
In vitro differentiation of satellite cells isolated from normal and dystrophic mammalian muscles. A comparison with embryonic myogenic cells.
Satellite cells were isolated from skeletal muscles of adult normal and dystrophic mice by sequential digestion of tissue fragments with collagenase, hyaluronidase and trypsin and are able to differentiate in culture and show appreciable differences as compared to their normal counterparts. Expand
Genotype control of the dystrophia muscularis gene in mice
This work has investigated how the process of myogenesis in vitro is affected not only by the two allelic mutants but also by the genetic background. Expand
Muscle Side Population Cells from Dystrophic or Injured Muscle Adopt a Fibro-Adipogenic Fate
It is indicated that muscle damage affects the lineage choices of muscle SP cells, promoting their differentiation along fibro-adipogenic lineages while inhibiting myogenesis, which has implications for a possible role of muscleSP cells in fibrosis and fat deposition in muscular dystrophy. Expand
Myogenic and chondrogenic cells.
This chapter focuses on the production of primary cultures from the embryonic muscle and cartilage with some reference to cell lines that may provide a useful alternative. Expand
Chapter 9 Cell Heterogeneity in The Myogenic Lineage
The evidence for the existence of different classes of myogenic cells, emerging at different periods during development and embryologically related to specific aspects of muscle differentiation and further maturation, is discussed. Expand
Isolation and characterization of an avian myogenic cell line.
The isolation and characterization of QM cell lines indicates that it may be feasible to isolate other avian myogenic cell lines with general utility for the study of muscle development and novel features of muscle gene expression in QM cells may prove useful for studying the role of specific muscle proteins during myogenesis. Expand
Thymic Myoid Cells as a Source of Cells for Myoblast Transfer
Evidence is presented that thymic myoid cells are capable of participating in the regeneration of postnatal skeletal muscle, resulting in the expression of donor-derived proteins such as dystrophin and retrovirally encoded proteins within host muscles, which leads to the conclusion that thye may provide an alternative to myoblasts derived from skeletal muscle as a source of myogenic cells for myoblast transfer. Expand


Normal and dystrophic chicken muscle at successive stages in tissue culture.
The histochemical features of normal and dystrophic cultures during successive stages of growth were compared and differences in the structure of myoblasts on young muscle fibers were examined. Expand
Failure of “Dystrophic” Neurones to Support Functional Regeneration of Normal or Dystrophic Muscle in Culture
THE traditional concept that muscular dystrophy is a “primary degenerative myopathy”1 has recently been challenged, and the suggestion put forward that the disease may in fact have a neural basis.Expand
Development of normal and genetically dystrophic mouse muscle in tissue culture. I. Prefusion and fusion activities of muscle cells: phase contrast and time lapse study.
  • J. Powell
  • Biology, Medicine
  • Experimental cell research
  • 1973
No differences were found between cultures of normal and dystrophic cells in their prefusion activities or in time of onset of rapid cell fusion, when initial concentrations of cells were kept constant. Expand
Expression of the dystrophia muscularis (dy) recessive gene in mice
A second strain BH WK ReJ (denoted dy2J/dy2J) was reported in 1970 which was described as being similar in all histopathological respects to the allelic dy/dy mutant except that it showed a slower progression of muscle necrosis. Expand
Morphological studies on normal and diseased human muscle in culture.
Abstract Normal and diseased human muscle, obtained by biopsy, has been successfully grown in tissue culture and has differentiated through multinucleate myoblast and myotube stages and has alsoExpand
The in vitro cultivation and differentiation capacities of myogenic cell lines.
Clonal analysis has shown that the capacity to differentiate is retained by the cells of all lines, but that there are differences in the expression of this capacity. Expand
Pathogenesis of muscular dystrophies. Sympathetic neurovascular components.
The clue to the pathogenesis of muscular dystrophies is believed to lie in a disorder of muscle. Recently, however, evidence is being presented that other myogenic factors might be involved. SeveralExpand
A myogenic cell line with altered serum requirements for differentiation.
  • D. Yaffe, O. Saxel
  • Biology, Medicine
  • Differentiation; research in biological diversity
  • 1977
Dfferentiation properties of a cell line, L84, which originated from a non-fusing clone isolated from the myogenic line L8, are described and show that L84 cells retain the programme for their differentiation into muscle fibres. Expand
Alterations of enzymatic activities during muscle differentiation in vitro.
The possibility that the messenger RNA molecules which specify the synthesis of proteins essential for cell fusion and increased enzymatic activity are formed at a developmental stage preceding cell fusion is suggested. Expand
A quantitative procedure for the dissociation of adult mammalian muscle into mononucleated cells.
A new technique for quantitative analysis of dissociated adult skeletal muscle is described, using adult hamster biceps, which obtained cell yields of 1–4 × 106 viable cells/g muscle and spontaneously contracting myotubes at day 7. Expand