Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle

  title={Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle},
  author={David Yaffe and O Saxel},
THE muscular dystrophies are a group of hereditary disorders manifested by a progressive wasting of the skeletal muscles. In spite of extensive studies, the nature of the primary lesion is unknown (for review see ref. 1). Because of the complex interaction between tissues, it is difficult to study this question in vivo. Therefore attempts have been made to investigate this question in cultures of dystrophic muscles of human or animal origin. Tissue explants as well as monolayer primary cell… 

A muscle cell line from dystrophic mice expressing an altered phenotype in vitro.

Because they can be grown in large amounts, and represent a pure muscle cell population which express an altered phenotype in an in vitro aneural avascular environment, DyA4 cells provide a very useful model system for investigating the pathogenesis of murine muscular dystrophy.

Tissue culture studies of muscle disorders: Part 2. Biochemical studies, nerve‐muscle culture, metabolic myopathies, and animal models

Study of protein, lipid, and purine metabolism of Duchenne muscular dystrophy cells in vitro and of muscle cells in combined culture with nerve cells find suggestions of altered collagen synthesis by DMD cells.

Genotype control of the dystrophia muscularis gene in mice

This work has investigated how the process of myogenesis in vitro is affected not only by the two allelic mutants but also by the genetic background.

Muscle Side Population Cells from Dystrophic or Injured Muscle Adopt a Fibro-Adipogenic Fate

It is indicated that muscle damage affects the lineage choices of muscle SP cells, promoting their differentiation along fibro-adipogenic lineages while inhibiting myogenesis, which has implications for a possible role of muscleSP cells in fibrosis and fat deposition in muscular dystrophy.

Myogenic and chondrogenic cells.

Thymic Myoid Cells as a Source of Cells for Myoblast Transfer

Evidence is presented that thymic myoid cells are capable of participating in the regeneration of postnatal skeletal muscle, resulting in the expression of donor-derived proteins such as dystrophin and retrovirally encoded proteins within host muscles, which leads to the conclusion that thye may provide an alternative to myoblasts derived from skeletal muscle as a source of myogenic cells for myoblast transfer.



Normal and dystrophic chicken muscle at successive stages in tissue culture.

The histochemical features of normal and dystrophic cultures during successive stages of growth were compared and differences in the structure of myoblasts on young muscle fibers were examined.

Failure of “Dystrophic” Neurones to Support Functional Regeneration of Normal or Dystrophic Muscle in Culture

THE traditional concept that muscular dystrophy is a “primary degenerative myopathy”1 has recently been challenged, and the suggestion put forward that the disease may in fact have a neural basis.

Expression of the dystrophia muscularis (dy) recessive gene in mice

A second strain BH WK ReJ (denoted dy2J/dy2J) was reported in 1970 which was described as being similar in all histopathological respects to the allelic dy/dy mutant except that it showed a slower progression of muscle necrosis.

Pathogenesis of muscular dystrophies. Sympathetic neurovascular components.

The clue to the pathogenesis of muscular dystrophies is believed to lie in a disorder of muscle. Recently, however, evidence is being presented that other myogenic factors might be involved. Several

A myogenic cell line with altered serum requirements for differentiation.

Dfferentiation properties of a cell line, L84, which originated from a non-fusing clone isolated from the myogenic line L8, are described and show that L84 cells retain the programme for their differentiation into muscle fibres.