Serial passage of MC3T3-E1 cells alters osteoblastic function and responsiveness to transforming growth factor-beta1 and bone morphogenetic protein-2.

@article{Chung1999SerialPO,
  title={Serial passage of MC3T3-E1 cells alters osteoblastic function and responsiveness to transforming growth factor-beta1 and bone morphogenetic protein-2.},
  author={Chi Y Chung and Akiko Iida-Klein and Lance E. Wyatt and George H. Rudkin and Koji Ishida and Dean T. Yamaguchi and Thomas A. Miller},
  journal={Biochemical and biophysical research communications},
  year={1999},
  volume={265 1},
  pages={246-51}
}
The murine-derived clonal MC3T3-E1 cell is a well-studied osteoblast-like cell line. To understand the effects of serial passages on its cellular function, we examined changes in cell morphology, gap junctional intercellular communication (GJIC), proliferation, and osteoblastic function between early passage (<20) and late passage (>65) cells. MC3T3-E1 cells developed an elongated, spindle shape after multiple passages. Intercellular communication decreased significantly (33%) in late vs. early… CONTINUE READING