Serial crystallography on in vivo grown microcrystals using synchrotron radiation

  title={Serial crystallography on in vivo grown microcrystals using synchrotron radiation},
  author={Cornelius Gati and Gleb Bourenkov and Marco Klinge and Dirk Rehders and Francesco Stellato and Dominik Oberthuer and Oleksandr Yefanov and Benjamin P. Sommer and Stefan Mogk and Michael Duszenko and Christian Betzel and Thomas R. Schneider and Henry N. Chapman and Lars Redecke},
Crystal structure determinations of biological macromolecules are limited by the availability of sufficiently sized crystals and by the fact that crystal quality deteriorates during data collection owing to radiation damage. Exploiting a micrometre-sized X-ray beam, high-precision diffractometry and shutterless data acquisition with a pixel-array detector, a strategy for collecting data from many micrometre-sized crystals presented to an X-ray beam in a vitrified suspension is demonstrated. By… CONTINUE READING
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