Serial Propagation of Influenza B (Lee) Virus in a Transmissible Line of Canine Kidney Cells

@article{Green1962SerialPO,
  title={Serial Propagation of Influenza B (Lee) Virus in a Transmissible Line of Canine Kidney Cells},
  author={Irving J. Green},
  journal={Science},
  year={1962},
  volume={138},
  pages={42 - 43}
}
  • I. Green
  • Published 5 October 1962
  • Biology, Medicine
  • Science
The serial propagation of influenza B (Lee) virus has been demonstrated in a transmissible line of canine kidney cells, as evidenced by the presence of cytopathic effects and hemagglutinins in each of six consecutive tissue-culture passages, and by egg infectivity titers of 10-4.3 and 10-4.1 per 0.1 ml in 3rd and 5th tissue culture fluids, respectively. 
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References

SHOWING 1-10 OF 13 REFERENCES
Isolation and Cytopathogenic Effect of Influenza B Viruses in Monkey Kidney Cultures.∗
Summary Influenza B viruses obtained in 1954 and 1955 have been isolated directly in cultures of monkey kidney. The latter system was shown to be preferable to the amniotic sac of the chick embryo
Growth Characteristics and Cytopathogenic Effects of Influenza A and B in Cultures of Human Embryo Tissues
TLDR
Recently isolated strains of influenza A and B have been propagated by serial passages in cultures of human embryo lung and kidney, with results of infectivity titrations higher in kidney cultures than in the amniotic sac, while the reverse was found with the influenza B virus propagated in lung cultures and titrated in the latter system.
The behavior of influenza viruses in various tissue culture systems.
Summary Comparison of the growth characteristics of first and twelfth monkey kidney (MK) passaged influenza B in the same type of culture indicated that adaptation had taken place after passage.
Primary Isolation of Influenza A, B, and G Viruses in Monkey Kidney Tissue Cultures.∗
  • K. Takemoto, R. Lynt, D. J. Davis
  • Biology, Medicine
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1955
TLDR
The use of monkey kidney cultures offered distinct advantages over chick embryos: (a) current strains of influenza B virus could be more readily isolated, (b) the time required for isolation and identification of the virus was shortened and (c) other respiratory tract viruses such as the Type 3 APC virus could been isolated.
Established Kidney Cell Lines of Normal Adult Bovine and Ovine Origin.∗
  • S. H. Madin, N. B. Darby
  • Biology, Medicine
    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
  • 1958
TLDR
Two newly established cell strains capable of continuous cultivation, one derived from adult bovine kidney and one from adult ovine kidney, are discussed and may be useful for those interested in veterinary virology in particular and virus-host relationships in general.
Perspectives In Virology (Wiley
  • New York,
  • 1959
Green, 0. C. Dierkhising, I. A. Phillips
  • Proc. Soc. Exptl. Biol. Med
  • 1955
Anti-Asian influenza serum obtained from E. H. Lennette, Viral and Rickettsial Disease Laboratory
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1
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