Serial Analysis of Gene Expression

@article{Velculescu1995SerialAO,
  title={Serial Analysis of Gene Expression},
  author={Victor E. Velculescu and Lichen Zhang and Bert Vogelstein and Kenneth W. Kinzler},
  journal={Science},
  year={1995},
  volume={270},
  pages={484 - 487}
}
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts… 
Serial analysis of gene expression.
  • K. Oien
  • Biology, Medicine
    Methods in molecular biology
  • 2003
Serial analysis of gene expression (SAGE™) is a patented, large-scale mRNA-profiling technology that produces comprehensive, quantitative, and reproducible gene expression profiles (originally
Serial Analysis of Gene Expression
TLDR
SAGE rests on the sequencing of short diagnostic tags, and as such increased by more than 1 order of magnitude the number of transcripts analyzed by current sequencing methods, making possible the quantification of low copy number mRNAs.
Serial analyses of gene expression (SAGE).
  • J. Dai
  • Biology, Medicine
    Methods in molecular medicine
  • 2005
TLDR
A detailed description is presented in this chapter of the procedures involved to prepare the SAGE libraries and protocols for automated sequencing can be found elsewhere, and thus are not included herein.
Serial analysis of gene expression: ESTs get smaller.
  • M. Adams
  • Biology, Medicine
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1996
TLDR
By coupling the strategy to current automated sequencing machines and the large expressed sequence tag databases, it should be possible to follow changes in gene expression for large numbers of genes economically and accurately.
Use of serial analysis of gene expression (SAGE) technology.
TLDR
An outline of the original method, several studies achieved by using the method as a major strategic tool, technological difficulties and intrinsic problems that emerged, and improvements and modifications of the method to cope with these drawbacks are presented.
Serial analysis of gene expression (SAGE).
TLDR
In this chapter, the SAGE procedure was described, which cannot only be used as a tool to generate gene expression profiles but also to identify new transcripts.
Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags.
TLDR
An RT-PCR-based method, rapid analysis of unknown SAGE tags (RAST-PCr), to analyse the expression of the corresponding genes and can be used as a screening method to investigate whether or not the gene is differentially expressed between several cell types of interest.
Serial analysis of gene expression (SAGE): advances, analysis and applications to pigment cell research.
TLDR
Serial analysis of gene expression (SAGE) allows for the expression profiling of the complete transcriptome of a given cell, and has the potential for identifying novel genes as well as those in low abundance.
Serial analysis of cereals
TLDR
SAGE libraries from developing wheat and germinating barley grains will provide an insight into the biological and physiological activity of cereal grains and the most useful high-throughput approach for gene profiling in terms of scale, economy and sensitivity.
Serial analysis of gene expression: from gene discovery to target identification.
TLDR
Serial Analysis of Gene Expression (SAGE) is a sequence-based genomics tool that features comprehensive gene discovery and quantitative gene expression capabilities that can characterize human disease at the molecular level while illuminating potential targets and markers for therapeutic and diagnostic development, respectively.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 21 REFERENCES
Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression
TLDR
The large–scale sequencing of a 3′–directed cDNA library from the human liver cell line HepG2, that is a non–biased representation of the mRNA population, is initiated, providing a complementary approach to structural analysis of the human genome by generating expressed sequence tags (ESTs).
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
TLDR
A method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction using a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer.
Selective expression of rat pancreatic genes during embryonic development.
  • J. Han, L. Rall, W. Rutter
  • Medicine, Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1986
TLDR
The data on gene expression suggest there are four developmental states of the exocrine pancreas: early morphogenesis and low-level gene expression (the protodifferentiated state), the embryonic differentiated state, a modulated state in neonatal animals, and the adult differentiated state.
Complementary DNA sequencing: expressed sequence tags and human genome project
TLDR
Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs), which will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing.
Sequence identification of 2,375 human brain genes
TLDR
2,672 new, independent cDNA clones isolated from four human brain cDNA libraries are partially sequenced to generate 2,375 expressed sequence tags to nuclear-encoded genes, representing an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome.
Gene expression profiles in normal and cancer cells.
TLDR
Analysis of gene expression patterns in gastrointestinal tumors provides insight into the extent of expression differences underlying malignancy and reveals genes that may prove useful as diagnostic or prognostic markers.
Identification of two distinct regulatory regions adjacent to the human β-interferon gene
To study the regulation of the human beta-interferon (beta-IFN) gene by poly(I)-poly(C), we analyzed the expression of deletion mutants of the cloned gene introduced into mouse cells on a new bovine
The GLI-Kruppel family of human genes.
TLDR
The GLI cDNA is used as a molecular probe to isolate related sequences from the human genome that probably control the expression of other genes and may be important in human development, tissue-specific differentiation, or neoplasia.
A molecular inventory of human pancreatic islets: sequence analysis of 1000 cDNA clones.
TLDR
The islet cDNA collection provides a unique source of genes for genetic studies of diabetes as well as for molecular studies of islet function in normal and diabetic states.
Isolation of cDNA clones encoding T cell-specific membrane-associated proteins
Of 10 distinct cloned DNA copies of mRNAs expressed in T lymphocytes but not in B lymphocytes and associated with membrane-bound polysomes, one hybridizes to a region of the genome that has
...
1
2
3
...