Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum

@article{Schrag1991SerHisGluTF,
  title={Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum},
  author={Joseph D. Schrag and Yunge Li and Shan Wu and Miroslaw Cygler},
  journal={Nature},
  year={1991},
  volume={351},
  pages={761-764}
}
THE Ser-His-Asp triad is a well known structural feature of the serine proteases. It has also been directly observed in the catalytic sites of two Upases, whose high-resolution three-dimensional structures have been determined1,2. Lipases show a wide variety of sizes, substrate and positional specificities, and catalytic rates3. They achieve maximal catalytic rates at oil–water interfaces. The fungus Geotrichum candidum produces several different forms of Upases, two of which have been purified… CONTINUE READING