Sequestration of fatty acids in triglycerides prevents endoplasmic reticulum stress in an in vitro model of cardiomyocyte lipotoxicity.

@article{Bosma2014SequestrationOF,
  title={Sequestration of fatty acids in triglycerides prevents endoplasmic reticulum stress in an in vitro model of cardiomyocyte lipotoxicity.},
  author={Madeleen Bosma and Dianne H. Dapito and Zoi Drosatos-Tampakaki and Ni Huiping-Son and L Huang and Sander Kersten and Konstantinos Drosatos and Ira J Goldberg},
  journal={Biochimica et biophysica acta},
  year={2014},
  volume={1841 12},
  pages={1648-55}
}
We used human cardiomyocyte-derived cells to create an in vitro model to study lipid metabolism and explored the effects of PPARγ; ACSL1 and ATGL on fatty acid-induced ER stress. Compared to oleate, palmitate treatment resulted in less intracellular accumulation of lipid droplets and more ER stress, as measured by upregulation of CHOP, ATF6 and GRP78 gene expression and phosphorylation of eukaryotic initiation factor 2a (EIF2a). Both ACSL1 and PPARγ adenovirus-mediated expression augmented… CONTINUE READING