Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae.

@article{Xie2015SequentialCO,
  title={Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae.},
  author={Wenping Xie and Lidan Ye and Xiaomei Lv and Haoming Xu and Hongwei Yu},
  journal={Metabolic engineering},
  year={2015},
  volume={28},
  pages={8-18}
}
Balanced utilization of metabolic intermediates and controllable expression of genes in biosynthetic pathways are key issues for the effective production of value-added chemicals in microbes. An inducer/repressor-free sequential control strategy regulated by glucose concentration in the growth environment was proposed to address these issues, and its efficiency was validated using heterologous beta-carotenoid biosynthesis in Saccharomyces cerevisiae as an example. Through sequential control of… CONTINUE READING